Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and <i>Varroa</i> Mites

Abstract Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence distribution Deformed wing virus (DWV), Acute bee paralysis (ABPV) Black queen cell (BQCV) infecting honey bees Varroa mites. Thirty mites feeding on 147 collected from twenty-five apiaries fifteen Elazığ. The samples subjected reverse transcriptase-polymerase chain reaction. All tested viruses found be present singly or as mixed infections. prevalence 23.81%, 12.93%, 10.20% for DWV, ABPV BQCV, respectively. Five out thirty infected only with DWV. Randomly chosen sequences each detected virus’s partial polyprotein gene region registered GenBank under accession numbers OP805878, OP805879, OP805880, OP805887, OP805888, OP805889, OP805890, OP805891 OP805881, OP805882, OP805883, OP805884 OP805885, OP805886 (BQCV). phylogenetic tree viral isolates compared world isolates. DWV closely related UK, Lebanon, Türkiye, France, Germany Israel isolates, whereas South Africa, Slovenia, Serbia, Hungary, Syria USA BQCV China, Korea, Czech Republic, Poland, Australia Sweden infections is revealed first time this study.